Laboratory for Biophysics (Bannai Lab)

Waseda University

Faculty of Science and Engineering

Department of Electrical Engineering and Bioscience
 

Please contact if you are interested in our research!

    • What's New

      2020.9.23
      Eight B3 students have been assigned to the Biophysics Lab. Welcome!

       

      2019.9.20
      List of 2020 publication

      1) Bannai H., Inoue T., Hirose M., Niwa F., Mikoshiba K. (2020) Synaptic Function and Neuropathological Disease Revealed by Quantum Dot-Single- Particle Tracking. In: Yamamoto N., Okada Y. (eds) Single Molecule Microscopy in Neurobiology. Neuromethods, vol 154. Humana, New York, NY.
      https://doi.org/10.1007/978-1-0716-0532-5_7


      2) Bannai H, Niwa F, Sakuragi S, Mikoshiba K.(2020) Inhibitory synaptic transmission tuned by Ca2+ and glutamate through the control of GABAA R lateral Diffusion dynamics.
      Dev Growth Differ (62) 398-406
      https://onlinelibrary.wiley.com/doi/full/10.1111/dgd.12667

       

      2019.9.25

      Presentation at the 57th Annual Meeting of the Biophysical Society of Japan Symposium on "Singularity Biology".

       

      2019.9.21
      Six B3 students have been assigned to the Biophysics Lab. These are the first students.

      2019.9.21

      Started a new Lab (Laboratory for Biophysics) in Waseda University, Faculty of Science and Engineering

      Department of Electrical Engineering and Bioscience, as a Professor


      2019.4.1

      Moved to Keio University, School of Medicine, Dept of Physiology, Yuzaki Lab, as a Designated Lecturer
      http://www.yuzaki-lab.org/?lang=en

      2019.3.23

      A video protocol for local Ca2+ imaging "Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators" has been published
      https://www.jove.com/video/59246/dissection-local-ca2-signals-cultured-cells-membrane-targeted-ca2

       

      2019.3.19

      Keynote lecture in The 16th International Membrane Research Forum
      https://groups.oist.jp/imrf

       

      2019.3.17

      Reported the research during the PRESTO single cell project.

      https://www.jst.go.jp/kisoken/presto/sympo/singlecell_20190317.pdf


      2018.12.8

      Lecture in 12th International Symposium on Nanomedicine in Yamaguchi


      2018.7.27
      Started "Singularity Biology" Project (MEXT Grant-in-Aid for Scientific Research on Innovative Areas.)

      http://singularity-bio.jp/


      2018.2.7
      Received 14th JSPS Prize
      http://www.riken.jp/en/pr/topics/2017/20171228_1/

    • Lab members

      FY2020 member

      Staff

      ○　HIROKO BANNAI (Professor)
      ○　SHIGEO SAKURAGI (Assistant Professor)

      ◯　KIMIKO TADA (Research Assistant)

      B4 (from EB)

      6 students

      ○　TAKAYA KAMEZAKI
      ◯　AYANO MACHIDA
      ◯　TOMOYA NOZAWA
      ○　TAKURO SHIOI ◯　TOMOYA SHOJI
      ◯　SHOTA TANIMOTO

      B3 (from EB)

      8 students

      ○ TBA

    • project PI

      Hiroko Bannai Ph. D

      Professor

      Working Address:

      Waseda University

      Faculty of Science and Engineering

      Department of Electrical Engineering and Bioscience
       

      2-2 Wakamatsu-cho, Shinjuku-ku,

      Tokyo 162-8480, Japan

       

      ResearcherID: I-7130-2014
      Publons: https://publons.com/a/1430791
      ORCID: http://orcid.org/0000-0002-0951-488X

      Research field

      Biophysics

      Neuroscience

      Cell Biology

      Research topics

      Single Molecule Imaging

      Neurons

      Glia

      Synaptic plasticity

      Calcium signals

    • REARCH

      Brain function revealed by single-molecule imaging

      QD-SPT: A powerful tool to visualize the molecular dynamics

      According to the fluid mosaic model, plasma membrane molecules such as lipids and transmembrane proteins have the ability to undergo lateral diffusion freely throughout the cell. In some cell types, however, specific membrane molecules are concentrated in cellular microdomains, by overcoming the randomizing effects of free diffusion. This polarized distribution of membrane molecules is crucial for various cell functions, thus it is important to understand the mechanism through which the cell regulates the lateral diffusion of membrane molecules.

       

      Quantum-dot single particle tracking (QD-SPT), a single molecule imaging technique using semiconductor nanocrystal quantum dots as a fluorescent probe, is a powerful tool to analyze the behavior of proteins and lipids on the plasma membrane. QD-SPT experiments that allowed us to obtain further insights into the strategy and physiological relevance of membrane self-organization in neurons and astrocytes, two major component cells in the brain.

      What does membrane dynamics tell us?

      Single-molecule resolution imaging has highlighted the existence and importance of self-organization mechanisms in neurons and glia. Additionally, a growing body of evidence demonstrates that neuronal and glial receptor dynamics become abnormal in disease states. Altered molecular diffusion dynamics comprise an important pathological phenotype.

    • FEATURED Publication

      Bannai H, Lévi S, Schweizer C, Dahan M, *Triller A. “Imaging the lateral diffusion of membrane molecules with quantum dots.”

      Nature Protocols 1:2628-2634. (2006)

      http://www.nature.com/nprot/journal/v1/n6/full/nprot.2006.429.html
       

      Bannai H, Lévi S, Schweizer C, Inoue T, Launey T. Racine V, Sibarita J.B, Mikoshiba K, Triller A.

      “Activity-Dependent Tuning of Inhibitory Neurotransmission Based on GABAAR Diffusion Dynamics”

      Neuron 62:670-682. (2009) * Selected for Cover

      http://www.sciencedirect.com/science/article/pii/S089662730900347X

       

      Arizono M, *Bannai H, Nakamura K, Niwa F, Enomoto M, Matsu-Ura T, Miyamoto A, Sherwood MW, Nakamura T, *Mikoshiba K. “Receptor-selective diffusion barrier enhances sensitivity of astrocytic processes to metabotropic glutamate receptor stimulation.”

      Science Signaling 5: ra27. (2012) *Featured at Science Signaling Pod Cast

       http://stke.sciencemag.org/content/5/218/ra27

       

      Bannai H1, Niwa F1, Sherwood MW, Shrivastava AN, Arizono M, Miyamoto A, Sugiura K, Lévi S, Triller A*, Mikoshiba K*. (1: co-first author) “Bidirectional Control of Synaptic GABAAR Clustering by Glutamate and Calcium”

      Cell Reports, 13: 2768-2780 (2015)

      http://www.cell.com/cell-reports/abstract/S2211-1247(15)01414-X

       

      REVIEW Article

      *Bannai H

      "Molecular membrane dynamics: Insights into synaptic function and neuropathological disease"

      Neuroscience Resaerch, 129: 47-56 (2018) * Selected for Cover

      https://www.sciencedirect.com/science/article/pii/S0168010217302274

    • Publication

      2020

      Bannai H., Inoue T., Hirose M., Niwa F., Mikoshiba K. (2020)

      "Synaptic Function and Neuropathological Disease Revealed by Quantum Dot-Single- Particle Tracking."

      In: Yamamoto N., Okada Y. (eds) Single Molecule Microscopy in Neurobiology. Neuromethods, vol 154. Humana, New York, NY.
      https://doi.org/10.1007/978-1-0716-0532-5_7


      Bannai H, Niwa F, Sakuragi S, Mikoshiba K.(2020)

      "Inhibitory synaptic transmission tuned by Ca2+ and glutamate through the control of GABAA R lateral Diffusion dynamics."

      Dev Growth Differ. (62) 398-406
      https://onlinelibrary.wiley.com/doi/full/10.1111/dgd.12667



      2019
      Bannai, H., Hirose, M., Niwa, F., Mikoshiba, K.

      "Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators."

       J. Vis. Exp. (145), e59246, doi:10.3791/59246 (2019).

      https://www.jove.com/video/59246/dissection-local-ca2-signals-cultured-cells-membrane-targeted-ca2


      2018

      *Bannai H (Review Article)

      "Molecular membrane dynamics: Insights into synaptic function and neuropathological disease"

      Neuroscience Resaerch, 129: 47-56 (2018) * Selected for Cover

      https://www.sciencedirect.com/science/article/pii/S0168010217302274

       


      2017

      Sakuragi S, Niwa F, Oda Y, Mikoshiba K*, Bannai H*

      “Astroglial Ca2+ signaling is generated by the coordination of IP3R and store-operated Ca2+ channels”

      Biochem Biophys Res Commun. 486: 879-85 (2017)

      http://www.sciencedirect.com/science/article/pii/S0006291X17305624

       

      Vervliet T, Pintelon I, Welkenhuyzen K, Bootman MD, Bannai H, Mikoshiba K, Martinet W, Nadif Kasri N, Parys JB, Bultynck G.

      Basal ryanodine receptor activity suppresses autophagic flux.

      Biochem Pharmacol.132:133-142. (2017) doi: 10.1016/j.bcp.2017.03.011.

      http://www.sciencedirect.com/science/article/pii/S0006295217301405

       

      Sherwood MW, Arizono M, Hisatsune C, Bannai H, Ebisui E, Sherwood JL, Panatier A, Oliet SH, Mikoshiba K.

      “Astrocytic IP3Rs: Contribution to Ca2+ signalling and hippocampal LTP.”

      Glia 65(3):502-513. (2017) doi: 10.1002/glia.23107.

      http://onlinelibrary.wiley.com/doi/10.1002/glia.23107/abstract

       

       

      2016

      Niwa F, Sakuragi S, Kobayashi A, Takagi S, Oda Y, Bannai H*, Mikoshiba K*.

      “Dissection of local Ca(2+) signals inside cytosol by ER-targeted Ca(2+) indicator.”

      Biochem Biophys Res Commun. 479(1):67-73 (2016)

      http://www.sciencedirect.com/science/article/pii/S0006291X16314851

       

      2015

      Bannai H1,, Niwa F1, Sherwood MW, Shrivastava AN, Arizono M, Miyamoto A, Sugiura K, Lévi S, Triller A*, Mikoshiba K*. (1: co-first author)

      “Bidirectional Control of Synaptic GABAAR Clustering by Glutamate and Calcium”

      Cell Reports, 13: 2768-2780 (2015)

      http://www.cell.com/cell-reports/abstract/S2211-1247(15)01414-X

       

      2014

      Arizono M, Bannai H, *Mikoshiba K.

      “Imaging mGluR5 Dynamics in Astrocytes Using Quantum Dots.”

      Curr Protoc Neurosci. 66:2.21.1-2.21.18. (2014)

      http://onlinelibrary.wiley.com/doi/10.1002/0471142301.ns0221s66/abstract

       

      Wu YW, Tang X, Arizono M, Bannai H, Shih PY, Dembitskaya Y, Kazantsev V, Tanaka M, Itohara S, Mikoshiba K, *Semyanov A. “Spatiotemporal calcium dynamics in single astrocytes and its modulation by neuronal activity.”

      Cell Calcium. 55:119-29. (2014)

      http://www.sciencedirect.com/science/article/pii/S0143416013001590

       

      2013

      Miyamoto A, Bannai H, Michikawa T, Mikoshiba K*.

      “Optimal microscopic systems for long-term imaging of intracellular calcium using a ratiometric genetically-encoded calcium indicator.”

      Biochem Biophys Res Commun. 434(2):252-7. (2013)

      http://www.sciencedirect.com/science/article/pii/S0006291X13004671

       

      2012

      Arizono M, *Bannai H, Nakamura K, Niwa F, Enomoto M, Matsu-Ura T, Miyamoto A, Sherwood MW, Nakamura T, *Mikoshiba K. “Receptor-selective diffusion barrier enhances sensitivity of astrocytic processes to metabotropic glutamate receptor stimulation.”

      Science Signaling 5: ra27. (2012)

            *Featured at Science Signaling Pod Cast

       http://stke.sciencemag.org/content/5/218/ra27

       

      Niwa F, *Bannai H, Arizono M, Fukatsu K, *Triller A, *Mikoshiba K “Gephyrin-independent GABAAR mobility and clustering during plasticity.”

          PLoS ONE 7: e36148. (2012)

      http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0036148

       

      Tamamushi S Nakamura T, Inoue T, Ebisui E, Sugiura K, Bannai H, *Mikoshiba K.

      “Type 2 inositol 1,4, 5-trisphosphate receptor is predominantly involved in agonist-induced Ca(2+) signaling in Bergmann glia. “

      Neurosci Res. 74: 32-41. (2012) * Selected for Cover

      http://www.sciencedirect.com/science/article/pii/S0168010212001393

       

      Nakamura H, Bannai H, Inoue T, *Michikawa T, Sano M, *Mikoshiba K. “Cooperative and stochastic calcium releases from multiple calcium puff sites generate calcium microdomains in intact HeLa cells.”

      J Biol Chem 287: 24563-24572. (2012)

       

      Renner M, Schweizer C, Bannai H, Triller A, *Lévi S.

      “Diffusion barriers constrain receptors at synapses.”

      PLoS ONE 7: e43032.(2012)

       

      ~2011

      Fukatsu K, Bannai H, InoueT, *Mikoshiba K.

      ”Lateral diffusion of inositol 1,4,5-trisphosphate receptor type 1 in Purkinje cells is regulated by calcium and actin filaments.”

      J Neurochem, 11: 1720-1733. (2010)

       

      Bannai H, Lévi S, Schweizer C, Inoue T, Launey T. Racine V, Sibarita J.B, Mikoshiba K, Triller A.

      “Activity-Dependent Tuning of Inhibitory Neurotransmission Based on GABAAR Diffusion Dynamics”

         Neuron 62:670-682. (2009) * Selected for Cover

      http://www.sciencedirect.com/science/article/pii/S089662730900347X

       

      Lévi S, Schweizer C, Bannai H, Pascual O, Charrier C, *Triller A.

      “Homeostatic regulation of synaptic GlyR numbers and lateral diffusion.”

      Neuron 59:261-273. (2008) [66, IF5y16.092]

       

      Bannai H, Lévi S, Schweizer C, Dahan M, *Triller A. “Imaging the lateral diffusion of membrane molecules with quantum dots.”

      Nature Protocols 1:2628-2634. (2006) [92, IF5y 11.296]

      http://www.nature.com/nprot/journal/v1/n6/full/nprot.2006.429.html

       

      Fukatsu K, Bannai H, *Inoue T, Mikoshiba K. “4.1N binding regions of inositol　1,4,5- trisphosphate receptor type 1.”

      Biochem Biophys Res Commun 342:573-576. (2006) [16 IF5y2.392]

       

      Tateishi Y, *Hattori M, Nakayama T, Iwai M, Bannai H, Nakamura T, Michikawa T, Inoue T, Mikoshiba K. “Cluster formation of inositol 1,4,5-trisphosphate receptor requires its transition to open state.”

        J Biol Chem 280:6816-6822. (2005)

       

      Bannai H, Fukatsu K, Mizutani A, Natsume T, Iemura SI, Ikegami T, *Inoue T, Mikoshiba K. “An RNA-interacting Protein, SYNCRIP (Heterogeneous Nuclear Ribonuclear Protein Q1/NSAP1) Is a Component of mRNA Granule Transported with Inositol 1,4,5- Trisphosphate Receptor Type 1 mRNA in Neuronal Dendrites.”

      J Biol Chem 279:53427-53434. (2004) [56]

       

      Fukatsu K, Bannai H, Zhang S, Nakamura H, *Inoue T, Mikoshiba K. “Lateral diffusion of inositol 1,4,5-trisphosphate receptor type 1 is regulated by actin filaments and 4.1N in neuronal dendrites.”

         J Biol Chem 279:48976-48982. (2004) [63]

       

      Bannai H, *Inoue T, Nakayama T, Hattori M, Mikoshiba K. “Kinesin dependent, rapid, bi-directional transport of ER sub-compartment in dendrites of hippocampal neurons.”

         J Cell Sci 117:163-175. (2004)

       

      Nakayama T, *Hattori M, Uchida K, Nakamura T, Tateishi Y, Bannai H, Iwai M, Michikawa T, Inoue T, Mikoshiba K. “The regulatory domain of the inositol 1,4,5- trisphosphate receptor is necessary to keep the channel domain closed: possible physiological significance of specific cleavage by caspase 3.”

      Biochem J 377:299-307. (2004)

       

      *Zhang S, Mizutani A, Hisatsune C, Higo T, Bannai H, Nakayama T, Hattori M, *Mikoshiba K. “Protein 4.1N is required for translocation of inositol 1,4,5-trisphosphate receptor type 1 to the basolateral membrane domain in polarized Madin-Darby canine kidney cells.”

         J Biol Chem 278:4048-4056. (2003)

       

      Bannai H, Yoshimura M, Takahashi K, *Shingyoji C. “Calcium regulation of microtubule sliding in reactivated sea urchin sperm flagella.”

         J Cell Sci 113: 831-839. (2000)

       

    • Movie

      【Movie】Mechanism of brain revealed by single-molecule imaging. (Japanese)

      2016, Aug, 6th Lecture to public.

    • Articles in RIKEN RESEARCH

       

       

      *Roaming receptors

      Neurons communicate more efficiently when neuronal activity causes inhibitory receptors to diffuse away from the synapse

       

       

      *A new starring role for astrocytes

      Identification of a novel membrane barrier in astrocytes may illuminate how neurological signaling is disrupted in patients with Alzheimer’s and epilepsy

    • Contact　連絡先

      Please fill out the following form and we will respond as soon as possible.

      2-2 Wakamatsucho, Shinjyuku, Tokyo, Japan
      hiroko.bannai@waseda.jp
      Submit

    • Bibliography　経歴

      Hiroko Bannai (Ph. D Sci)

      Education

      1995-2000

      Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan.

      PhD of Science: March 29th, 2000; Master of Science: March 28th, 1997.

       

      1991-1995

      Department of Biological Sciences, Faculty of Science, University of Tokyo

      Bachelor of Science: March 28th, 1995

      Research

      2019 September-
      Professor

      Waseda University, Faculty of Science and Engineering, Dept of Electrical Engineering and Bioscience,

      2019 April –2019 September

      Designated Lecturer
      Dept of Neurophysiology, Keio University School of Medicine

      2016 April –2019 March

      JST PRESTO researcher (Full-time) /RIKEN Visiting Research Scientist

      “Innovative technology platforms for integrated single cell analysis”

       

      2013 April –2015 March

      Designated Lecturer

      Lab. Brain Function and Structure (Nagoya Research Center for Brain & Neural Circuits)

      Department of Biological Science

      Graduate School of Science, Nagoya University, Japan

      PRESTO researcher (concurrent position) 2015 October – 2016 March

      “Innovative technology platforms for integrated single cell analysis”

       

      2012 April –2013 March

      Postdoctoral fellow, JSPS research fellow

      Division of Molecular Neurobiology, Brain Science Institute, RIKEN.

      Supervisor: Dr. Katsuhiko MIKOSHIBA

       

      2010 April –2012 March

      Research Scientist

      Division of Molecular Neurobiology, Brain Science Institute, RIKEN.

      Supervisor: Dr. Katsuhiko MIKOSHIBA

       

      2007 April -2010 March

      Special Postdoctoral Researcher

      Division of Molecular Neurobiology, Brain Science Institute, RIKEN.

      Supervisor: Dr. Katsuhiko MIKOSHIBA

       

      2005 April -2007 March

      Postdoctoral fellow, JSPS research fellow,

      Biologie Cellulaire de la Synapse N & P, INSERM U789

      Ecole Normale Supérieure Paris, France

      Supervisor: Dr Antoine TRILLER

       

      2000 April -2005 March

      Research Scientist

      Division of Molecular Neurobiology, Brain Science Institute, RIKEN.

      Supervisor: Dr. Katsuhiko MIKOSHIBA

       

      1995 April -2000 March

      Ph.D Student

      Graduate School of Science, University of Tokyo.

      Supervisor: Dr. Chikako SHINGYOJI

      Teaching

      2013-2015

      Physiology I for the third-year undergraduate students.

      (1-2 classes among 14 classes in a semester, in Japanese)

       

      2014-2015

      A seminar class for the second-year undergraduate students.

      (6-7 classes among 14 classes in a semester, in Japanese)

       

      2013-2014

      Laboratory training for the second-year undergraduate students.

      (in Japanese and in English)

       

      2014-2015

      Supervise a Post-doctoral researcher (Dr. Shigeo Sakuragi)

       

      2007-2012

      Supervise two Ph. D students

      (Univ. Tokyo, Dr. Misa Arizono and Dr. Fumihiro Niwa).

       

      2002-2005

      Supervise a Ph. D student (Univ. Tokyo, Dr. Kazumi Fukatsu).

       

      1996-1998

      Teaching assistant, University of Tokyo.

      Awards

      2017

      14th JSPS Prize

       

      2013

      Japan Neuroscience Society Young Investigator Award

       

      2010

      RIKEN SPDR Poster award

       

      2008

      4th Early Research in Biophysics Award of the Biophysical Society of Japan

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