• What's New

    2020.9.23
    Eight B3 students have been assigned to the Biophysics Lab. Welcome!

     

    2019.9.20
    List of 2020 publication

    1) Bannai H., Inoue T., Hirose M., Niwa F., Mikoshiba K. (2020) Synaptic Function and Neuropathological Disease Revealed by Quantum Dot-Single- Particle Tracking. In: Yamamoto N., Okada Y. (eds) Single Molecule Microscopy in Neurobiology. Neuromethods, vol 154. Humana, New York, NY.
    https://doi.org/10.1007/978-1-0716-0532-5_7


    2) Bannai H, Niwa F, Sakuragi S, Mikoshiba K.(2020) Inhibitory synaptic transmission tuned by Ca2+ and glutamate through the control of GABAA R lateral Diffusion dynamics.
    Dev Growth Differ (62) 398-406
    https://onlinelibrary.wiley.com/doi/full/10.1111/dgd.12667

     

    2019.9.25

    Presentation at the 57th Annual Meeting of the Biophysical Society of Japan Symposium on "Singularity Biology".

     

    2019.9.21
    Six B3 students have been assigned to the Biophysics Lab. These are the first students.

    2019.9.21

    Started a new Lab (Laboratory for Biophysics) in Waseda University, Faculty of Science and Engineering

    Department of Electrical Engineering and Bioscience, as a Professor


    2019.4.1

    Moved to Keio University, School of Medicine, Dept of Physiology, Yuzaki Lab, as a Designated Lecturer
    http://www.yuzaki-lab.org/?lang=en

    2019.3.23

    A video protocol for local Ca2+ imaging "Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators" has been published
    https://www.jove.com/video/59246/dissection-local-ca2-signals-cultured-cells-membrane-targeted-ca2

     

    2019.3.19

    Keynote lecture in The 16th International Membrane Research Forum
    https://groups.oist.jp/imrf

     

    2019.3.17

    Reported the research during the PRESTO single cell project.

    https://www.jst.go.jp/kisoken/presto/sympo/singlecell_20190317.pdf


    2018.12.8

    Lecture in 12th International Symposium on Nanomedicine in Yamaguchi


    2018.7.27
    Started "Singularity Biology" Project (MEXT Grant-in-Aid for Scientific Research on Innovative Areas.)

    http://singularity-bio.jp/


    2018.2.7
    Received 14th JSPS Prize
    http://www.riken.jp/en/pr/topics/2017/20171228_1/

  • Lab members

    FY2020 member

    Staff

    ○ HIROKO BANNAI (Professor)
    ○ SHIGEO SAKURAGI (Assistant Professor)

    ◯ KIMIKO TADA (Research Assistant)

    B4 (from EB)

    6 students

    ○ TAKAYA KAMEZAKI
    ◯ AYANO MACHIDA
    ◯ TOMOYA NOZAWA

    ○ TAKURO SHIOI ◯ TOMOYA SHOJI
    ◯ SHOTA TANIMOTO

    B3 (from EB)

    8 students

    ○ TBA
  • project PI

    Hiroko Bannai Ph. D

    Professor

    Working Address:

    Waseda University

    Faculty of Science and Engineering

    Department of Electrical Engineering and Bioscience
     

    2-2 Wakamatsu-cho, Shinjuku-ku,

    Tokyo 162-8480, Japan

     

    Research field

    Biophysics

    Neuroscience

    Cell Biology

    Research topics

    Single Molecule Imaging

    Neurons

    Glia

    Synaptic plasticity

    Calcium signals

  • REARCH

    Brain function revealed by single-molecule imaging

    QD-SPT: A powerful tool to visualize the molecular dynamics

    According to the fluid mosaic model, plasma membrane molecules such as lipids and transmembrane proteins have the ability to undergo lateral diffusion freely throughout the cell. In some cell types, however, specific membrane molecules are concentrated in cellular microdomains, by overcoming the randomizing effects of free diffusion. This polarized distribution of membrane molecules is crucial for various cell functions, thus it is important to understand the mechanism through which the cell regulates the lateral diffusion of membrane molecules.

     

    Quantum-dot single particle tracking (QD-SPT), a single molecule imaging technique using semiconductor nanocrystal quantum dots as a fluorescent probe, is a powerful tool to analyze the behavior of proteins and lipids on the plasma membrane. QD-SPT experiments that allowed us to obtain further insights into the strategy and physiological relevance of membrane self-organization in neurons and astrocytes, two major component cells in the brain.

    What does membrane dynamics tell us?

    Single-molecule resolution imaging has highlighted the existence and importance of self-organization mechanisms in neurons and glia. Additionally, a growing body of evidence demonstrates that neuronal and glial receptor dynamics become abnormal in disease states. Altered molecular diffusion dynamics comprise an important pathological phenotype.

  • FEATURED Publication

    Bannai H, Lévi S, Schweizer C, Dahan M, *Triller A. “Imaging the lateral diffusion of membrane molecules with quantum dots.”

    Nature Protocols 1:2628-2634. (2006)

    http://www.nature.com/nprot/journal/v1/n6/full/nprot.2006.429.html
     

    Bannai H, Lévi S, Schweizer C, Inoue T, Launey T. Racine V, Sibarita J.B, Mikoshiba K, Triller A.

    “Activity-Dependent Tuning of Inhibitory Neurotransmission Based on GABAAR Diffusion Dynamics”

    Neuron 62:670-682. (2009) * Selected for Cover

    http://www.sciencedirect.com/science/article/pii/S089662730900347X

     

    Arizono M, *Bannai H, Nakamura K, Niwa F, Enomoto M, Matsu-Ura T, Miyamoto A, Sherwood MW, Nakamura T, *Mikoshiba K. “Receptor-selective diffusion barrier enhances sensitivity of astrocytic processes to metabotropic glutamate receptor stimulation.”

    Science Signaling 5: ra27. (2012) *Featured at Science Signaling Pod Cast

     http://stke.sciencemag.org/content/5/218/ra27

     

    Bannai H1, Niwa F1, Sherwood MW, Shrivastava AN, Arizono M, Miyamoto A, Sugiura K, Lévi S, Triller A*, Mikoshiba K*. (1: co-first author) “Bidirectional Control of Synaptic GABAAR Clustering by Glutamate and Calcium”

    Cell Reports, 13: 2768-2780 (2015)

    http://www.cell.com/cell-reports/abstract/S2211-1247(15)01414-X

     

    REVIEW Article

    *Bannai H

    "Molecular membrane dynamics: Insights into synaptic function and neuropathological disease"

    Neuroscience Resaerch, 129: 47-56 (2018) * Selected for Cover

    https://www.sciencedirect.com/science/article/pii/S0168010217302274

  • Publication

    2020

    Bannai H., Inoue T., Hirose M., Niwa F., Mikoshiba K. (2020)

    "Synaptic Function and Neuropathological Disease Revealed by Quantum Dot-Single- Particle Tracking."

    In: Yamamoto N., Okada Y. (eds) Single Molecule Microscopy in Neurobiology. Neuromethods, vol 154. Humana, New York, NY.
    https://doi.org/10.1007/978-1-0716-0532-5_7


    Bannai H, Niwa F, Sakuragi S, Mikoshiba K.(2020)

    "Inhibitory synaptic transmission tuned by Ca2+ and glutamate through the control of GABAA R lateral Diffusion dynamics."

    Dev Growth Differ. (62) 398-406
    https://onlinelibrary.wiley.com/doi/full/10.1111/dgd.12667



    2019
    Bannai, H., Hirose, M., Niwa, F., Mikoshiba, K.

    "Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators."

     J. Vis. Exp. (145), e59246, doi:10.3791/59246 (2019).

    https://www.jove.com/video/59246/dissection-local-ca2-signals-cultured-cells-membrane-targeted-ca2


    2018

    *Bannai H (Review Article)

    "Molecular membrane dynamics: Insights into synaptic function and neuropathological disease"

    Neuroscience Resaerch, 129: 47-56 (2018) * Selected for Cover

    https://www.sciencedirect.com/science/article/pii/S0168010217302274

     


    2017

    Sakuragi S, Niwa F, Oda Y, Mikoshiba K*, Bannai H*

    “Astroglial Ca2+ signaling is generated by the coordination of IP3R and store-operated Ca2+ channels”

    Biochem Biophys Res Commun. 486: 879-85 (2017)

    http://www.sciencedirect.com/science/article/pii/S0006291X17305624

     

    Vervliet T, Pintelon I, Welkenhuyzen K, Bootman MD, Bannai H, Mikoshiba K, Martinet W, Nadif Kasri N, Parys JB, Bultynck G.

    Basal ryanodine receptor activity suppresses autophagic flux.

    Biochem Pharmacol.132:133-142. (2017) doi: 10.1016/j.bcp.2017.03.011.

    http://www.sciencedirect.com/science/article/pii/S0006295217301405

     

    Sherwood MW, Arizono M, Hisatsune C, Bannai H, Ebisui E, Sherwood JL, Panatier A, Oliet SH, Mikoshiba K.

    “Astrocytic IP3Rs: Contribution to Ca2+ signalling and hippocampal LTP.”

    Glia 65(3):502-513. (2017) doi: 10.1002/glia.23107.

    http://onlinelibrary.wiley.com/doi/10.1002/glia.23107/abstract

     

     

    2016

    Niwa F, Sakuragi S, Kobayashi A, Takagi S, Oda Y, Bannai H*, Mikoshiba K*.

    “Dissection of local Ca(2+) signals inside cytosol by ER-targeted Ca(2+) indicator.”

    Biochem Biophys Res Commun. 479(1):67-73 (2016)

    http://www.sciencedirect.com/science/article/pii/S0006291X16314851

     

    2015

    Bannai H1,, Niwa F1, Sherwood MW, Shrivastava AN, Arizono M, Miyamoto A, Sugiura K, Lévi S, Triller A*, Mikoshiba K*. (1: co-first author)

    “Bidirectional Control of Synaptic GABAAR Clustering by Glutamate and Calcium”

    Cell Reports, 13: 2768-2780 (2015)

    http://www.cell.com/cell-reports/abstract/S2211-1247(15)01414-X

     

    2014

    Arizono M, Bannai H, *Mikoshiba K.

    “Imaging mGluR5 Dynamics in Astrocytes Using Quantum Dots.”

    Curr Protoc Neurosci. 66:2.21.1-2.21.18. (2014)

    http://onlinelibrary.wiley.com/doi/10.1002/0471142301.ns0221s66/abstract

     

    Wu YW, Tang X, Arizono M, Bannai H, Shih PY, Dembitskaya Y, Kazantsev V, Tanaka M, Itohara S, Mikoshiba K, *Semyanov A. “Spatiotemporal calcium dynamics in single astrocytes and its modulation by neuronal activity.”

    Cell Calcium. 55:119-29. (2014)

    http://www.sciencedirect.com/science/article/pii/S0143416013001590

     

    2013

    Miyamoto A, Bannai H, Michikawa T, Mikoshiba K*.

    “Optimal microscopic systems for long-term imaging of intracellular calcium using a ratiometric genetically-encoded calcium indicator.”

    Biochem Biophys Res Commun. 434(2):252-7. (2013)

    http://www.sciencedirect.com/science/article/pii/S0006291X13004671

     

    2012

    Arizono M, *Bannai H, Nakamura K, Niwa F, Enomoto M, Matsu-Ura T, Miyamoto A, Sherwood MW, Nakamura T, *Mikoshiba K. “Receptor-selective diffusion barrier enhances sensitivity of astrocytic processes to metabotropic glutamate receptor stimulation.”

    Science Signaling 5: ra27. (2012)

          *Featured at Science Signaling Pod Cast

     http://stke.sciencemag.org/content/5/218/ra27

     

    Niwa F, *Bannai H, Arizono M, Fukatsu K, *Triller A, *Mikoshiba K “Gephyrin-independent GABAAR mobility and clustering during plasticity.”

        PLoS ONE 7: e36148. (2012)

    http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0036148

     

    Tamamushi S Nakamura T, Inoue T, Ebisui E, Sugiura K, Bannai H, *Mikoshiba K.

    “Type 2 inositol 1,4, 5-trisphosphate receptor is predominantly involved in agonist-induced Ca(2+) signaling in Bergmann glia. “

    Neurosci Res. 74: 32-41. (2012) * Selected for Cover

    http://www.sciencedirect.com/science/article/pii/S0168010212001393

     

    Nakamura H, Bannai H, Inoue T, *Michikawa T, Sano M, *Mikoshiba K. “Cooperative and stochastic calcium releases from multiple calcium puff sites generate calcium microdomains in intact HeLa cells.”

    J Biol Chem 287: 24563-24572. (2012)

     

    Renner M, Schweizer C, Bannai H, Triller A, *Lévi S.

    “Diffusion barriers constrain receptors at synapses.”

    PLoS ONE 7: e43032.(2012)

     

    ~2011

    Fukatsu K, Bannai H, InoueT, *Mikoshiba K.

    ”Lateral diffusion of inositol 1,4,5-trisphosphate receptor type 1 in Purkinje cells is regulated by calcium and actin filaments.”

    J Neurochem, 11: 1720-1733. (2010)

     

    Bannai H, Lévi S, Schweizer C, Inoue T, Launey T. Racine V, Sibarita J.B, Mikoshiba K, Triller A.

    “Activity-Dependent Tuning of Inhibitory Neurotransmission Based on GABAAR Diffusion Dynamics”

       Neuron 62:670-682. (2009) * Selected for Cover

    http://www.sciencedirect.com/science/article/pii/S089662730900347X

     

    Lévi S, Schweizer C, Bannai H, Pascual O, Charrier C, *Triller A.

    “Homeostatic regulation of synaptic GlyR numbers and lateral diffusion.”

    Neuron 59:261-273. (2008) [66, IF5y16.092]

     

    Bannai H, Lévi S, Schweizer C, Dahan M, *Triller A. “Imaging the lateral diffusion of membrane molecules with quantum dots.”

    Nature Protocols 1:2628-2634. (2006) [92, IF5y 11.296]

    http://www.nature.com/nprot/journal/v1/n6/full/nprot.2006.429.html

     

    Fukatsu K, Bannai H, *Inoue T, Mikoshiba K. “4.1N binding regions of inositol 1,4,5- trisphosphate receptor type 1.”

    Biochem Biophys Res Commun 342:573-576. (2006) [16 IF5y2.392]

     

    Tateishi Y, *Hattori M, Nakayama T, Iwai M, Bannai H, Nakamura T, Michikawa T, Inoue T, Mikoshiba K. “Cluster formation of inositol 1,4,5-trisphosphate receptor requires its transition to open state.”

      J Biol Chem 280:6816-6822. (2005)

     

    Bannai H, Fukatsu K, Mizutani A, Natsume T, Iemura SI, Ikegami T, *Inoue T, Mikoshiba K. “An RNA-interacting Protein, SYNCRIP (Heterogeneous Nuclear Ribonuclear Protein Q1/NSAP1) Is a Component of mRNA Granule Transported with Inositol 1,4,5- Trisphosphate Receptor Type 1 mRNA in Neuronal Dendrites.”

    J Biol Chem 279:53427-53434. (2004) [56]

     

    Fukatsu K, Bannai H, Zhang S, Nakamura H, *Inoue T, Mikoshiba K. “Lateral diffusion of inositol 1,4,5-trisphosphate receptor type 1 is regulated by actin filaments and 4.1N in neuronal dendrites.”

       J Biol Chem 279:48976-48982. (2004) [63]

     

    Bannai H, *Inoue T, Nakayama T, Hattori M, Mikoshiba K. “Kinesin dependent, rapid, bi-directional transport of ER sub-compartment in dendrites of hippocampal neurons.”

       J Cell Sci 117:163-175. (2004)

     

    Nakayama T, *Hattori M, Uchida K, Nakamura T, Tateishi Y, Bannai H, Iwai M, Michikawa T, Inoue T, Mikoshiba K. “The regulatory domain of the inositol 1,4,5- trisphosphate receptor is necessary to keep the channel domain closed: possible physiological significance of specific cleavage by caspase 3.”

    Biochem J 377:299-307. (2004)

     

    *Zhang S, Mizutani A, Hisatsune C, Higo T, Bannai H, Nakayama T, Hattori M, *Mikoshiba K. “Protein 4.1N is required for translocation of inositol 1,4,5-trisphosphate receptor type 1 to the basolateral membrane domain in polarized Madin-Darby canine kidney cells.”

       J Biol Chem 278:4048-4056. (2003)

     

    Bannai H, Yoshimura M, Takahashi K, *Shingyoji C. “Calcium regulation of microtubule sliding in reactivated sea urchin sperm flagella.”

       J Cell Sci 113: 831-839. (2000)

     

  • Movie

    【Movie】Mechanism of brain revealed by single-molecule imaging. (Japanese)

    2016, Aug, 6th Lecture to public.

  • Articles in RIKEN RESEARCH

     

     

    *Roaming receptors

    Neurons communicate more efficiently when neuronal activity causes inhibitory receptors to diffuse away from the synapse

     

     

    *A new starring role for astrocytes

    Identification of a novel membrane barrier in astrocytes may illuminate how neurological signaling is disrupted in patients with Alzheimer’s and epilepsy

  • Contact 連絡先

    Please fill out the following form and we will respond as soon as possible.

    2-2 Wakamatsucho, Shinjyuku, Tokyo, Japan
  • Bibliography 経歴

    Hiroko Bannai (Ph. D Sci)

    Education

    1995-2000

    Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan.

    PhD of Science: March 29th, 2000; Master of Science: March 28th, 1997.

     

    1991-1995

    Department of Biological Sciences, Faculty of Science, University of Tokyo

    Bachelor of Science: March 28th, 1995

    Research

    2019 September-
    Professor

    Waseda University, Faculty of Science and Engineering, Dept of Electrical Engineering and Bioscience,

    2019 April –2019 September

    Designated Lecturer
    Dept of Neurophysiology, Keio University School of Medicine

    2016 April –2019 March

    JST PRESTO researcher (Full-time) /RIKEN Visiting Research Scientist

    “Innovative technology platforms for integrated single cell analysis”

     

    2013 April –2015 March

    Designated Lecturer

    Lab. Brain Function and Structure (Nagoya Research Center for Brain & Neural Circuits)

    Department of Biological Science

    Graduate School of Science, Nagoya University, Japan

    PRESTO researcher (concurrent position) 2015 October – 2016 March

    “Innovative technology platforms for integrated single cell analysis”

     

    2012 April –2013 March

    Postdoctoral fellow, JSPS research fellow

    Division of Molecular Neurobiology, Brain Science Institute, RIKEN.

    Supervisor: Dr. Katsuhiko MIKOSHIBA

     

    2010 April –2012 March

    Research Scientist

    Division of Molecular Neurobiology, Brain Science Institute, RIKEN.

    Supervisor: Dr. Katsuhiko MIKOSHIBA

     

    2007 April -2010 March

    Special Postdoctoral Researcher

    Division of Molecular Neurobiology, Brain Science Institute, RIKEN.

    Supervisor: Dr. Katsuhiko MIKOSHIBA

     

    2005 April -2007 March

    Postdoctoral fellow, JSPS research fellow,

    Biologie Cellulaire de la Synapse N & P, INSERM U789

    Ecole Normale Supérieure Paris, France

    Supervisor: Dr Antoine TRILLER

     

    2000 April -2005 March

    Research Scientist

    Division of Molecular Neurobiology, Brain Science Institute, RIKEN.

    Supervisor: Dr. Katsuhiko MIKOSHIBA

     

    1995 April -2000 March

    Ph.D Student

    Graduate School of Science, University of Tokyo.

    Supervisor: Dr. Chikako SHINGYOJI

    Teaching

    2013-2015

    Physiology I for the third-year undergraduate students.

    (1-2 classes among 14 classes in a semester, in Japanese)

     

    2014-2015

    A seminar class for the second-year undergraduate students.

    (6-7 classes among 14 classes in a semester, in Japanese)

     

    2013-2014

    Laboratory training for the second-year undergraduate students.

    (in Japanese and in English)

     

    2014-2015

    Supervise a Post-doctoral researcher (Dr. Shigeo Sakuragi)

     

    2007-2012

    Supervise two Ph. D students

    (Univ. Tokyo, Dr. Misa Arizono and Dr. Fumihiro Niwa).

     

    2002-2005

    Supervise a Ph. D student (Univ. Tokyo, Dr. Kazumi Fukatsu).

     

    1996-1998

    Teaching assistant, University of Tokyo.

    Awards

    2017

    14th JSPS Prize

     

    2013

    Japan Neuroscience Society Young Investigator Award

     

    2010

    RIKEN SPDR Poster award

     

    2008

    4th Early Research in Biophysics Award of the Biophysical Society of Japan